Effect of Specimen Anticoagulant and Storage on Mea- Surement of Serum and Plasma Fatty Acid

References 1. Adult Treatment Panel II. Summary of the second report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel II). JAMA 1993;269:3015–23. 2. Havel R, Eder H, Bragdon J. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest 1955;34:1345–53. 3. Friedewald WT, Levy RJ, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin Chem 1972;18:499–502. 4. Bachorik PS. Measurement of low-density lipoprotein cholesterol. In: Rifai N, Warnick GR, Dominiczak MH, eds. Handbook of lipoprotein testing. Washington, DC: AACC Press, 1997:145–60. 5. Bachorik PS, Ross JW. National Cholesterol Education Program recommendations for measurement of low-density lipoprotein cholesterol: executive summary. Clin Chem 1995;41:1414–20. 6. McNamara JR, Cole TG, Contois JH, Ferguson CA, Ordovas JM, Schaefer EJ. Immunoseparation method for measuring low-density lipoprotein cholesterol directly from serum evaluated. Clin Chem 1995;41:232–40. 7. Okabe H, Nakamura M. Homogeneous LDL-cholesterol methods. Development, principle, standardization and certification. Fats of Life 1999;XIII:1– 13. 8. Nauck M, Graziani MS, Bruton D, Cobbaert C, Cole TG, Lefevre F, et al. Analytical and clinical performance of a detergent-based homogeneous LDL-cholesterol assay: a multicenter evaluation. Clin Chem 2000;46:506– 14. 9. Nauck M, Rifai N. Analytical performance and clinical efficacy of three routine procedures for LDL cholesterol measurement compared with the ultracentrifugation-dextran sulfate-Mg method. Clin Chim Acta 2000;294:77–92. 10. Esteban-Salán M, Guimón-Bardesi A, de la Viuda-Unzueta JM, Azcárate-Ania MN, Pascual-Usandizaga P, Amoroto-Del Rı́o E. Analytical and clinical evaluation of two homogeneous assays for LDL-cholesterol in hyperlipidemic patients. Clin Chem 2000;46:1121–31. 11. Passing H, Bablok W. Comparison of several regression procedures for method comparison studies and determination of sample sizes. Application of linear regression procedures for method comparison studies in clinical chemistry. Part II. J Clin Chem Clin Biochem 1984;22:431–5. 12. Sugiuchi H, Irie T, Uji Y, Ueno T, Chaen T, Uekama K, Okabe H. Homogeneous assay for measuring low-density lipoprotein cholesterol in serum with triblock copolymer and a-cyclodextrin sulfate. Clin Chem 1998;44:522–31. 13. Lackner KJ, Schmitz G. b-VLDL of patients with type III hyperlipoproteinemia interferes with homogeneous determination of HDL-cholesterol based on polyethylene glycol-modified enzymes. Clin Chem 1998;44:2546–8. 14. Escolá-Gil JC, Jorba O, Julve-Gil J, González-Sastre F, Ordóñez-Llanos J, Blanco-Vaca F. Pitfalls of direct HDL-cholesterol measurements in mouse models of hyperlipidemia and atherosclerosis. Clin Chem 1999;45:1567–9. 15. Sugiuchi H, Uji Y, Okabe H, Irie T, Uekama K, Kayahara N, Miyauchi K. Direct measurement of high-density lipoprotein cholesterol in serum with polyethylene glycol modified enzymes and sulfated a-cyclodextrin. Clin Chem 1995;41:717–23. 16. Seman LJ, Jenner JL, McNamara JR, Schaefer EJ. Quantification of lipoprotein-(a) in plasma by assaying cholesterol in lectin-bound plasma fraction. Clin Chem 1994;40:400–3. 17. Seman LJ, DeLuca C, Jenner JL, Cupples A, McNamara JR, Wilson PWF, et al. Lipoprotein(a)-cholesterol and coronary heart disease in the Framingham Heart Study. Clin Chem 1999;45:1039–46. Effect of Specimen Anticoagulant and Storage on Measurement of Serum and Plasma Fatty Acid Ethyl Ester Concentrations, Raneem O. Salem, Joanne E. Cluette-Brown, Ali Hasaba, and Michael Laposata (Division of Laboratory Medicine, Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114; * Address correspondence to this author at: Room 235, Gray Bldg., Massachusetts General Hospital, Boston, MA 02114; fax 617-726-3256, e-mail mlaposata@ partners.org)